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How to Detect Growth Hormones?

Lois

Want to build muscle faster than the rest of the world? Need to make weight in a hurry? Visit the sports nutrition stores, and they’ll set you up with all the ingredients you need to put on size. Bring along your billfold and be ready for a little extra cash in your pocket. Growth hormones are natural chemical substances that regulate and speed up growth. They are also known as anabolic steroids, the term given to drugs with similar effects, produced mainly for performance-enhancing purposes, and many people prefer to wachstumshormone kaufen.

Growth hormones often have very potent effects on a person’s body. There is no risk of irreversible damage since growth hormone levels return to normal once the treatment ends. However, they can have harmful effects such as increased sexual desire or emotional instability. The doping control procedures used by the World Anti-Doping Agency (WADA) are effective for detecting synthetic growth hormones through urine and blood tests.

Ways to Detect Growth Hormones

To detect growth hormones, their origin, and the substance they are contained, you should use a gas chromatography-mass spectrometry (GC-MS) test. It is the ideal method for detecting synthetic growth hormones and their analogs, peptides, and proteins. The WADA has also created a specific list of substances to carry out this test (WADA 2012). The list currently consists of at least 90 substances and is updated regularly.

To test the presence of growth hormones in a person’s body using GC-MS, you will need, A chromatography with an electron multiplier interface; – A mass spectrometer (sector detector); An ion-specific detector; A detector, and a data system programmed with software. The procedure is as follows. 

  • The first thing to do is prepare a solution containing growth hormone or its analog. The mixture can contain a high concentration of growth hormone and other low-molecular-weight compounds.
  • The sample should be submitted to a process of extraction. First, the solution is extracted with a solvent that dissolves inorganic salts, sugars, and similar biomolecules.
  • The solution is heated to around 100 degrees, which dissolves any proteins present in the mixture.
  • The protein solution is filtered through a 0. 45-m two paper filter and left to cool. The paper filter will trap any proteins present in the solution when it is subjected to heating and will also help concentrate the molecule in the solution. Next, a supercritical fluid extraction is performed on the solution containing proteins.

  • The mixture of proteins, salts, and sugars is sent through a gun for washing, containing a solvent that immerses 100% of metabolites contained in the mixture. Then, the mixture is injected into the chromatograph with an electron multiplier interface.
  • The mixture is manipulated to ensure that the extraction is explicitly performed. It is done using a rotary electric field (EEF).

The process of the chromatograph begins with the injection of the mixture into the first chamber of a chromatograph. The compounds dissolving will then be sent through a column of stationary phase, which contains an extractant that will separate dissolved molecules based on their charge and molecular weight. Try to stay focused to understand better how to detect growth hormones.